Figure 2.

¹⁵N-HSQC spectrum of τ_C14. The spectrum was recorded at 30°C with a 1.8 mM solution of uniformly ¹⁵N/¹³C-labeled τ_C14 in 90% H₂O/10% D₂O at pH 6.8 in phosphate buffer (10 mM phosphate, 100 mM NaCl, 1.0 mM dithiothreitol and 0.1 mM NaN₃). The cross-peaks are assigned using one-letter amino acid symbols and residue numbers. Side-chain resonances are identified by Greek characters. Cross-peaks from side-chain amides are connected by horizontal lines.