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. 2007 Apr 22;35(9):2825–2832. doi: 10.1093/nar/gkm080

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Changes in amide chemical shifts of ¹⁵N-labeled τ_C16Δ11 observed upon binding of α at pH 6.8 and 25°C. The data were recorded with concentrations of τ_C16Δ11 and α of 90 and 120 μM, respectively. The chemical shift change Δδ was calculated as ({[Δδ(¹H)]² + [0.1Δδ(¹⁵N)]²}/2)^1/2, where Δδ(¹H) and Δδ(¹⁵N) denote the chemical shift changes in p.p.m. observed in the ¹H and ¹⁵N dimensions of the ¹⁵N-HSQC spectrum. The locations of the α-helices and β-strands in τ_C14 are indicated by open and filled bars, respectively.