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. 2007 Apr 22;35(9):3118–3127. doi: 10.1093/nar/gkm168

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Resolution of dPTP, S_P and R_P-diastereoisomers of dPTPαS by rp-HPLC. Trace a shows the rp-HPLC of the dPTPαS racemate after DEAE-Sephadex chromatography. Trace b shows the purified racemate with dPTP added as a reference to indicate that any trace of dPTP can be readily separated from the diastereoisomers of dPTPαS by rp-HPLC. Traces c and d show the pure diastereomers of dPTPαS following rp-HPLC; these are the materials that were used in polymerase extension studies. Peaks: 1, S isomer; 2, R isomer; 3, dPTP.