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. 2007 Apr 11;35(9):2846–2860. doi: 10.1093/nar/gkm155

Figure 2.

Figure 2.

(A) Absorption spectra of free BMVC and its complexes with H24 in K+ solution upon BMVC titration. (B) The job plots of BMVC to the H24 in Na+ and in K+ solutions obtained from (A). (C) Fluorescence titration of 10 nM BMVC by adding H24 from 0.25 to 12 nM in K+ solution. The inset showed the binding plots of γ versus Cf for the titration. The fitting parameters to the equation, γ = nKCf/(1 + KCf), are K = 1.12 × 109 with n ∼ 2.4 in Na+ solution, and K = 1.07 × 109 mol−1 with n ∼ 2.3 in K+ solution.