Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Apr 10;35(9):e67. doi: 10.1093/nar/gkm193

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Conditional shRNA expression from H1 promoter variants. TetR-expressing cells were transfected with 100 ng pCMV-β-gal, 800 ng pGL3-control (Promega) and 100 ng anti-luc shRNA expressing test plasmids under control of various H1 promoter variants. Several different tetR-expressing cell lines were used. (A) SK-N-BE (2) TR, (B) SJNB-8 TR, (C) IMR32 TR, (D) SK-N-AS TR, (E) HeLa TR, (F) HEK293-T REx (Invitrogen). NC: negative control; 100 ng pCMV-β-gal and 800 ng pGL3-basic (no luciferase expression), PC: positive control; 100 ng pCMV-β-gal and 800 ng pGL3-control (constitutive luciferase expression), + indicates addition of 1 μg/ml dox 1-day post-transfection. Cells were incubated for a total of 3 days. Error bars indicate SDs.