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. 2007 Apr 16;35(9):2924–2935. doi: 10.1093/nar/gkm126

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Loop II mutants participate in BER. Lane 1 is the annealed 33-mer oligonucleotide that was the starting material; lane 2 is the 33-mer oligonucleotide that was treated with UDG; lane 3 is UDG-treated 16-mer substrate incubated with whole-cell extract from Δβ-MEF. The solid arrow points to the radiolabeled 16-mer that is the product of base removal and incision by UDG and APE I, respectively. The dotted arrow points to the 17-mer, resulting from n + 1 addition by pol β. The dashed arrow near the top points to ligated product, which is 33 nt in length. Lanes 4–7 are incubation for 1 min with Δβ-MEF. Lanes 8–11 are incubation for 2 min with Δβ-MEF. Lanes 12–15 are incubation for 5 min with Δβ-MEF. Lanes 16–19 are incubation for 10 min with Δβ-MEF. Lanes 20–23 are incubation for 20 min with Δβ-MEF. Lanes 4, 8, 12, 16 and 20 contain wild-type pol β. Lanes 5, 9, 13, 17 and 21 contain Loopless. Lanes 6, 10, 14, 18 and 22 is 9-Ala. Lanes 7, 11, 15, 19 and 23 contain 5-Ala. The fraction of 16-mer that was repaired is below each lane.