Figure 7.

Lambda-loop, 5-Ala and Loop1- are mutators in the missing base primer extension assay in the absence of dCTP. Quantification of the products for all loop II variants was performed using Phosphoimager and ImageQuant software as described in the Materials and methods section. (A) nucleotide pool missing A; (B) nucleotide pool missing T; (C) nucleotide pool missing C; (D) nucleotide pool missing G. The X-axis lists the loop mutants. 1 is 9-Ala, 2 is Loopless, 3 is 5-Ala, 4 is 4-Ala, 5 is 3-Ala, 6 is 2-Ala, 7 is 1-Ala, 8 is Loop1-, 9 is lambda-loop and 10 is Loop2-. Loop mutants are organized in groups of 10 for each graph with the incubation time points of 2, 5 and 10 min listed underneath. Y-axis is the fold-increase of loop II variant product over wild-type product. The results are the average of three experiments. Error bars represent the standard deviation of the mean.