Table 1.

Primers for site-directed mutagenesis

Primer	Sequence
Loopless FW	5′ TGTTTGCCAGCTTCCC_CACAGGAGAATCGATATC 3′
Loopless RV	5′ GATATCGATTCTCCTGTG_GGGAAGCTGGCAAACA 3′
9-Ala Loop FW	5′ GGGTGTTTGCCAGCTTCCCGCGGCGGCGGCGGCGGCGGCGGCGGCGCACAGGAGAATCGATATCAGG 3′
9-Ala Loop RV	5′ CCTGATATCGATTCTCCTGTGCGCCGCCGCCGCCGCCGCCGCCGCCGCGGGAAGCTGGCAAACACCC 3′
5-Ala Loop FW	5′ TTTGCCAGCTTCCCGCGGCGGCGGCGGCGCACAGGAGAATCGATATC 3′
5-Ala Loop RV	5′ ATCGATTCTCCTGTGCGCCGCCGCCGCCGCGGGAAGCTGGCAAACA 3′
4-Ala Loop FW	5′ TGTTTGCCAGCTTCCCGCGGCGGCGGCGCACAGGAGAATCGATATC 3′
4-Ala Loop RV	5′ GATATCGATTCTCCTGTGCGCCGCCGCCGCGGGAAGCTGGCAAACA 3′
3-Ala Loop FW	5′ GTTTGCCAGCTTCCCGCGGCGGCGCACAGGAGAATCG 3′
3-Ala Loop RV	5′ CGATTCTCCTGTGCGCCGCCGCGGGAAGCTGGCAAAC 3′
2-Ala Loop FW	5′ GGTGTTTGCCAGCTTCCCGCGGCGCACAGGAGAATCGATATC 3′
2-Ala Loop RV	5′ GATATCGATTCTCCTGTGCGCCGCGGGAAGCTGGCAAACACC 3′
1-Ala Loop FW	5′ GGGTGTTTGCCAGCTTCCCGCGCACAGGAGAATCGATATCAG 3′
1-Ala Loop RV	5′ CTGATATCGATTCTCCTGTGCGCGGGAAGCTGGCAAACACCC 3′
Loop1-FW	5′ GTGTTTGCCAGCTTCCC_GAAAACGAATATCCACACA 3′
Loop1-RV	5′ TGTGTGGATATTCGTTTTC_GGGAAGCTGGCAAACAC 3′
Lambda Loop FW	5′ TGTTTGCCAGCTTCCCGGTCCGGGTAAACGTCACAGGAGAATCGATATC 3′
Lambda Loop RV	5′ GATATCGATTCTCCTGTGACGTTTACCCGGACCGGGAAGCTGGCAAACA 3′
Loop2-FW	5′ CTTCCCAGCGAGAATGAT_CACAGGAGAATCGATATC 3′
Loop2-RV	5′ GATATCGATTCTCCTGTG_ATCATTCTCGCTGGGAAG 3′

The underlined nucleotides in insertion primers (9-Ala, 5-Ala, 4-Ala, 3-Ala, 2-Ala, 1-Ala and Lambda-loop) indicate the nucleotides inserted into loopless sequence. The underlined space in deletion primers (Loopless, Loop1-, Loop2-)indicates where the deletion occurred in wild-type sequence.