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. 2007 Apr 16;35(9):2924–2935. doi: 10.1093/nar/gkm126

Table 2.

DNA duplex constructs for burst and fidelity assays

DNA substrate Sequence
Burst substrate 45T-22U-22D 5′GCCTCGCAGCCGTCCAACCAAC CAACCTCGATCCAATGCCGTCC CGGAGCGTCGGCAGGTTGGTTGAGTTGGAGCTAGGTTACGGCAGG 3′
Gap substrate CII45-CIIU-CIID 5′TTGCGACTTATCAACGCCCACA    AGCT GTCTT CTCAGTT CC AACGCTGAATAGTTGCGGGT GTAGTCATCGACAGAAGAGTCAAGG 3′