Table 3.
Presteady-state and steady-state constants
| Loop constructs | Amino acid sequence | Presteady-state burst constant (s−1) | Steady-state constant (s−1) | |
|---|---|---|---|---|
| Wild type | QLP SENDENEYP | HR | 14.5 ± 5.93 | 0.422 |
| Loopless | QLP | HR | No burst | 0.0394 ± 0.0010 |
| 9-Ala Loop | QLPAAAAAAAAA | HR | 12.2 ± 2.55 | 0.386 |
| 5-Ala Loop | QLPAAAAA | HR | 12.0 ± 4.34 | 1.16 |
| 4-Ala Loop | QLPAAAA | HR | 12.2 ± 1.71 | 1.02 |
| 3-Ala Loop | QLPAAA | HR | 11.0 ± 1.98 | 0.343 |
| 2-Ala Loop | QLPAA | HR | No burst | 0.599 ± 0.080 |
| 1-Ala Loop | QLPA | HR | No burst | 0.407 ± 0.044 |
| Loop1- | QLPENEYP | HR | 16.8 ± 4.99 | 1.01 |
| Lambda Loop | QLP GPGKR | HR | 7.58 ± 0.589 | 0.739 |
| Loop2- | QLPSEND | HR | 14.5 ± 4.99 | 0.457 |