Table 4.
HSV-tk forward mutation frequency of wild-type and five residue loop II mutants
| Polymerase | Viable cells (per ml) CmR | Mutant cells (per ml) CmR FUdRR | HSV-tk mutant frequency (10−4) | HSV-tk mutant frequency increase over WT |
|---|---|---|---|---|
| DNA pol β WT | 68 800 | 160 | 23.2 | |
| 25 800 | 100 | 38.7 | ||
| 25 200 | 100 | 39.6 | ||
| Mean (SD) | 33.8 (9.22) | |||
| 5-Ala | 28 200 | 760 | 269.5 | |
| 40 800 | 1080 | 264.7 | ||
| 60 000 | 1200 | 200 | ||
| Mean (SD) | 245 (38.8) | 7.24 | ||
| Lambda-loop | 62 040 | 820 | 132 | |
| 45 840 | 680 | 148.3 | ||
| 98 700 | 1100 | 111.4 | ||
| Mean (SD) | 130 (18.6) | 3.84 | ||
| Loop1- | 17 760 | 400 | 225 | |
| 12 420 | 340 | 273 | ||
| 19 800 | 600 | 303 | ||
| Mean (SD) | 267 (39.3) | 7.90 |