Table 1.

Primers and probes for TaqMan® QPCR

Gene	Name	Sequence
RassF1aa	Methylated forward	5′GCGTTGAAGTCGGGGTTC3′
	Methylated reverse	5′CCCGTACTTCGCTAACTTTAAACG3′
	Methylated probe	6FAM-5′ACAAACGCGAACCGAACGAAACCA3-TAMRA
	Unmethylated forward	5′GTGTTGAAGTTGGGGTTT3′
	Unmethylated reverse	5′CCCATACTTCACTAACTTTAAACA3′
	Unmethylated probe I	6FAM-5′-ACAAACACAAACCAAACAAAACCA-3′-TAMRA
	Unmethylated probe II	HEX-5′-ACAAACACAAACCAAACAAAACCA-3′-BHQ1
GstP1b	Methylated forward	5′TTCGGGGTGTAGCGGTCGTC3′
	Methylated reverse	5′GCCCCAATACTAAATCACGACG3′
	Methylated probe	6FAM-5′TAAAAAATCCCGCGAACTCCCGC3′-TAMRA
	Unmethylated forward	5′GATGTTTGGGGTGTAGTGGTTGTT3′
	Unmethylated reverse	5′CCACCCCAATACTAAATCACAACA3′
	Unmethylated probe	6FAM-5′AAAAATCCCACAAACTCCCACC3′-TAMRA
APCc	Methylated forward	5′GAACCAAAACGCTCCCCAT3′
	Methylated reverse	5′TTATATGTCGGTTACGTGCGTTTATAT3′
	Methylated probe	6FAM-5′CCCGTCGAAAACCCGCCGATTA3′-TAMRA
	Unmethylated forward	5′CTAAATACAAACCAAAACACTCCCCAT3′
	Unmethylated reverse	5′AGTTATATGTTGGTTATGTGTGTTTATAT3′
	Unmethylated probe I	6FAM-5′CCCATCAAAAACCCACCAATTA3′-TAMRA
	Unmethylated probe II	CY5-5′CCCATCAAAAACCCACCAATTA3′-BHQ3
	Unconverted forward	5′GGACCAGGGCGCTCCCCAT3′
	Unconverted reverse	5′CCACATGTCGGTCACGTGCGCCCACAC3′
	Unconverted probe	6FAM-5′CCCGTCGGGAGCCCGCCGATTG3′-TAMRA
Rarβd	Methylated forward	5′AGAACGCGAGCGATTCGAGTAG3′
	Methylated reverse	5′TACAAAAAACCTTCCGAATACGTT3′
	Methylated probe	6FAM-5′ATCCTACCCCGACGATACCCAAAC3′-TAMRA
	Unmethylated forward	5′TTGAGAATGTGAGTGATTTGAGTAG3′
	Unmethylated reverse	5′TTACAAAAAACCTTCCAAATACATTC3′
	Unmethylated probe	6FAM-5′AAATCCTACCCCAACAATACCCAAAC3′-TAMRA

^aRassF1a: Homo sapiens Ras association (Ra1GDS/AF-6) domain family 1, (RassF1), transcript variant A (34).

^bGstP1: Glutathione S-transferase pi (35,46).

^cAPC: Human adenomatous polyposis coli (APC) gene (36).

^dRarβ: Human retinoic acid receptor beta gene (37); TAMRA: Carboxytetramethylrhodamine; FAM: 6-Carboxyfluorescein; HEX: 6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein; CY5: Indodicarbocyanine; BHQ1: 4′-(2-Nitro-4-toluyldiazo)-2′-methoxy-5′-methyl-diazobenzene-4′-(N-ethyl)-N-2-O-ethanol; and BHQ3: 3-Diethylamino-5-phenylphenazium-7-diazobenzene-4″-(N-ethyl)-N-2-O-ethanol.