Table 2.
Overall duplex recoveries APC and GstP1 (60 838 input copies)
| Cell line | Gene | Methylation state | Observed copies | M/U + M | U + M | Total recovery |
|---|---|---|---|---|---|---|
| PC3 | APC | Methylated | 3175 ± 1905* | 0.984 | 3225 | 0.053 |
| Unmethylated | <50 | |||||
| GstP1 | Methylated | 837 ± 567 | 0.208 | 4017 | 0.066 | |
| Unmethylated | 3180 ± 1700 | |||||
| HK293 | APC | Methylated | <50 | <0.0159 | 3153 | 0.051 |
| Unmethylated | 3103 ± 1805 | |||||
| GstP1 | Methylated | <50 | <0.0294 | 1703 | 0.028 | |
| Unmethylated | 1653 ± 1003 |
*Recoveries at 200 ng input DNA (predicted to contain 60 838 copies) ±S.D. based on five independent measurements.