Table 3.
Extension templates for extension and blunt-end cloning
| Gene | Name | Sequence |
|---|---|---|
| RassF1a | Methylated top | 5′GCGTTGAAGTCGGGGTTCGTTTTGTGGTTTCGTTCGGTTCGCGTTTGT3′ |
| Methylated bottom | 5′CCCGTACTTCGCTAACTTTAAACGCTAACAAACGCGAACCGAACGAAA3′ | |
| Unmethylated top | 5′GTGTTGAAGTTGGGGTTTGTTTTGTGGTTTTGTTTGGTTTGTGTTTGT3′ | |
| Unmethylated bottom | 5′CCCATACTTCACTAACTTTAAACACTAACAAACACAAACCAAACAAAA3′ | |
| GstP1 | Methylated top | 5′TTCGGGGTGTAGCGGTCGTCGGGTTGGGGTCGGCGGGAGTTCGCGGGATTTTTTAGA3′ |
| Methylated bottom | 5′GCCCCAATACTAAATCACGACGCCGACCGCTCTTCTAAAAAATCCCGCGAACTCCC3′ | |
| Unmethylated top | 5′GATGTTTGGGGTGTAGTGGTTGTTGGGTTGGGGTTGGTGGGAGTTTGTGGGATTTTTT3′ | |
| Unmethylated bottom | 5′CCACCCCAATACTAAATCACAACACCAACCACTCTTCTAAAAAATCCCACAAACTCCC3′ | |
| APC | Methylated top | 5′GAACCAAAACGCTCCCCATTCCCGTCGAAAACCCGCCGATTAACTAA3′ |
| Methylated bottom | 5′TTATATGTCGGTTACGTGCGTTTATATTTAGTTAATCGGCGGGTTTT3′ | |
| Unmethylated top | 5′CTAAATACAAACCAAAACACTCCCCATTCCCATCAAAAACCCACCAATTAAC3′ | |
| Unmethylated bottom | 5′AGTTATATGTTGGTTATGTGTGTTTATATTTAGTTAATTGGTGGGTTTTTGA3′ | |
| Rarβ | Methylated top | 5′AGAACGCGAGCGATTCGAGTAGGGTTTGTTTGGGTATCGTCGGGGTAGGA3′ |
| Methylated bottom | 5′TACAAAAAACCTTCCGAATACGTTCCGAATCCTACCCCGACGATACCCAA3′ | |
| Unmethylated top | 5′TTGAGAATGTGAGTGATTTGAGTAGGGTTTGTTTGGGTATTGTTGGGGTAGG3′ | |
| Unmethylated bottom | 5′TTACAAAAAACCTTCCAAATACATTCCAAATCCTACCCCAACAATACCCAAA3′ |