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Determination of the affinity of L20C-ter for its mRNA- and rRNA-binding sites. The fluorescence anisotropy titration curves for wild-type and mutant operator RNAs are shown in (A). The equivalent titration curves for the 23S rRNA are shown in (B) where the left and right ordinates are for anisotropy measurements performed with 23S rRNA and wild-type operator RNA, respectively.
