Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Distances between Cy3 and Cy5 in different constructs
| Construct | % Efficiency | Cy3–Cy5 distance (Å) |
|---|---|---|
| WT | 14 ± 1 | 73 ± 1 |
| Δ4W | 13 ± 1 | 74 ± 1 |
| ΔLOOP | 9.4 ± 1 | 79 ± 2 |
| ΔU74 | 13 ± 1 | 74 ± 1 |
| ΔUG | 12 ± 1 | 75 ± 1 |
The invariant top strand was labeled with Cy3 and Cy5 on 5′ end and 3′ end respectively. In each construct, by paring the 32-nt labeled oligomer with its bottom strand, the distance between the two dyes was measured by the acceptor normalization method described in the text and shown in Figure 5B.
