Figure 2.

ATPase activity of the wild-type and different deletion mutant proteins. ATPase assay using various HpDnaB forms. Enzyme-linked ATPase assays were performed using protocol as described in the materials and methods either in the absence or presence of DNA. The rates of the reactions against different substrate (ATP) concentrations were plotted for HpDnaBWt, HpDnaBDelN1, HpDnaBDelN2 and HpDnaBDelN3 as shown in panels A, C, E and G, respectively. Lineweaver-Burk plots of the same enzymes were also plotted either in the absence or presence of DNA in each case (panels B, D, F and H, respectively). Maximum velocity of the reaction (Vmax) and turn-over number (Kcat) were calculated in each case (as shown in Table 2).