Table 1.
Primers list
| No | Primer name | Sequence |
|---|---|---|
| 1 | HpDnaBWt(Fw) | 5′-GCGGATCCATGGATCATTTAAAGCATTTGC-3′ |
| 2 | HpDnaBWt(Rv) | 5′-GCGGATCCTCAAGTTGTAACTATATCATAA-3′ |
| 3 | HpDnaBmut(Fw) | 5′-GTCATTATAGGGGCATGCCCGTCTATGGGTAAA-3′ |
| 4 | HpDnaBmut(Rv) | 5′-TTTACCCATAGACGGGCATGCCCCTATAATGAC-3′ |
| 5 | HpDnaBDelN1(Fw) | 5′-CGGGATCCATGCGCCAAAAAATGCCTAAAGAC-3′ |
| 6 | HpDnaBDelN1(Rv) | same as HpDnaBWt(Rv) |
| 7 | HpDnaBDelN2(Fw) | 5′-CGGGATCCATGGTGGAGCGAGAAGTCTATG-3′ |
| 8 | HpDnaBDelN2(Rv) | same as HpDnaBWt(Rv) |
| 9 | HpDnaBDelN3(Fw) | 5′-CGGGATCCGAAGTTACTGGCATACCG-3′ |
| 10 | HpDnaBDelN3(Rv) | same as HpDnaBWt(Rv) |
| 11 | HpDnaBDelN4 (Fw) | 5′-CGGGATCCATGGGGAGTTTAGTCATTATAGG-3′ |
| 12 | HpDnaBDelN4 (Rv) | same as HpDnaBWt(Rv) |
| 13 | HpDnaBDelC1(Fw) | same as HpDnaBWt(Fw) |
| 14 | HpDnaBDelC1 (Rv) | 5′-CGGGATCCTCAAGCGTTAAAGCGCGTATAAAC-3′ |
| 15 | HpDNABDel34(Fw) | 5′-CAAATGAGGGCTGAAAGCATTGAAGAGGCT-3′ |
| 16 | HpDnaBDel34(Rv) | 5′-AGCCTCTTCAATGCTTTCAGCCCTCATTTG-3′ |