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. 2000 Dec 5;97(26):14168–14171. doi: 10.1073/pnas.011518098

Figure 1.

Figure 1

Time course of polyP-dependent AMP phosphorylation. The reaction mixtures (100 μl) containing 50 mM Tris⋅HCl (pH 8.0), 50 mM ammonium sulfate, 10 mM MgCl2, 5 mM AMP, polyP (75 mM of phosphate, chain length, 15–20), 4 μg purified PPK, and a cell lysate (0.8 μg of proteins) was incubated at 30°C. The ADP and ATP formed were monitored and plotted as the sum of both levels. ■, No lysate; ⧫, the cell lysate of JM109 harboring the control vector pTrc99A; ●, the cell lysate of JM109 overproducing ADK.