Figure 2.

Autoradiogram showing phosphotransfer from [³²P]polyP to AMP by the combined action of both purified enzymes. Each or both of 4 μg purified PPK and ADK were used for enzyme samples in the PAP assay with [³²P]polyP. Each sample was analyzed by PEI-TLC followed by autoradiography: lanes 1 and 6, reaction compounds with no enzyme; lanes 2 and 7, reaction compounds containing PPK; lanes 3 and 8, reaction compounds containing ADK; lanes 4, 5, and 9, reaction compounds containing PPK and ADK; lanes 1–4, AMP was omitted from the reaction mixtures. Other details are given in Materials and Methods.