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A stoichiometry of PPK and ADK required for the PAP activity. The purified PPK (25 pmol) and various doses of the purified ADK (1.3, 2.5, 6.3, 13, 25, and 63 pmol) were used for enzyme samples in the PAP assay. The relative activity to the value observed for 25 pmol of both enzymes was plotted against the dose of ADK added, the value of which was expressed relative to the amount of PPK.
