Figure 3.

(A) HPLC traces of a time course of 0.5 mM benzoyl-CoA hydrolysis by 0.25 μM wild-type HMWP1 at 22°C. The control reaction excluding HMWP1 was performed in parallel. (B) HPLC traces of the hydrolysis of 0.5 mM benzoyl-CoA by 1 μM wild-type HMWP1 and its various single-point Ser → Ala mutants for 120 min at 22°C. (C) HPLC traces of the hydrolysis of 1 mM acetyl-CoA by 0.5 μM wild-type and various single-point Ser → Ala mutants of HMWP1, and the PCP₃-TE fragment for 20 min at 22°C. The HPLC traces were shifted progressively by 0.5 min for A and C and 0.4 min for B.