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Kinetic parameters of acyl-SNAC hydrolysis at 22°C
| Enzyme | Substrate | kcat, min−1 | Km, μM | kcat/Km, mM−1⋅min−1 | Relative activity |
|---|---|---|---|---|---|
| PCP3-TE | HPT-SNAC | 47 ± 16 | 1,700 ± 689 | 27.6 | 31 |
| HMWP1 | HPT-SNAC | 17 ± 7 | 1,730 ± 905 | 9.8 | 11 |
| HMWP1 | HPTT-SNAC | 0.24 ± 0.03 | 265 ± 59 | 0.9 | 1 |
