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Substrate specificity of acyl-CoAs to wild-type HMWP1
| Substrate | kcat, min−1 | Km, μM | kcat/Km, mM−1⋅min−1 | Relative activity |
|---|---|---|---|---|
| Malonyl-CoA | 6.2 ± 0.1 | 3,290 ± 107 | 1.9 | 1 |
| Methylmalonyl-CoA | 4.3 ± 0.2 | 4,379 ± 436 | 0.98 | 0.5 |
| Acetyl-CoA | 142 ± 5 | 435 ± 47 | 326 | 171.6 |
| Acetoacetyl-CoA | 5 ± 1 | 2,442 ± 1,036 | 2.1 | 1.1 |
| Decanoyl-CoA | 2.1 ± 0.2 | 356 ± 123 | 5.9 | 3.1 |
| Benzoyl-CoA | 8.1 ± 0.1 | 85 ± 4 | 95.3 | 50.2 |
| Phenylacetyl-CoA | 4.6 ± 0.3 | 108 ± 28 | 42.6 | 22.4 |
