Table 3.

Hydrolytic rates of 1 mM acetyl-CoA and 0.5 mM benzoyl-CoA by various enzymes

Enzyme	kobs, min−1
	Acetyl-CoA	Benzoyl-CoA
PCP3-TE	0.29	0
HMWP1 (1–1895)	ND	0.19
HMWP1 (1812–3163)	ND	0.2
HMWP1 (1896–3163)	ND	0
HMWP1 (wild-type)	98.95	6.94
HMWP1 (TE mutant)	0.33	0.04
HMWP1 (AT mutant)	2.53	0.14
HMWP1 (ACP mutant)	7.52	0.17
HMWP1 (PCP3 mutant)	4.37	0.15
AT mutant/TE mutant	2.47	ND
AT mutant/ACP mutant	8.45	ND
AT mutant/PCP3 mutant	5.7	ND

Enzyme concentrations were 0.5 μM for acetyl-CoA and 1 μM for benzoyl CoA. ND, not determined.