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. 2000 Dec 19;97(26):14200–14205. doi: 10.1073/pnas.97.26.14200

Figure 3.

Figure 3

Depletion of hTFIIIB50 from a partially purified fraction (cEDF 0.2) using antibodies against either hTFIIIB90 or hTFIIIB50. (A) Ten microliters of cEDF 0.2 (lane 1), preimmune control antibody flow-through (FT, lane 2), anti-hTFIIIB90 antibody flow-through (lane 3), or anti-hTFIIIB90 eluate (lane 4) was separated by SDS/12.5% PAGE and transferred onto nitrocellulose. A 1:10,000 dilution of anti-hTFIIIB50 antibody in PBS-0.05% Tween 20 and 5% skim milk powder was used for Western blot analyses that were performed as described (8). (B) Ten microliters of cEDF 0.2 (lane 1), anti-hTFIIIB50 antibody flow-through from cEDF 0.2 (lane 2) or anti-hTFIIIB50 eluate from cEDF 0.2 (lane 3) was separated by SDS/12.5% PAGE and transferred onto nitrocellulose. SDS/PAGE and Western blot procedures were described (A; ref. 8).