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. 2000 Dec 19;97(26):14200–14205. doi: 10.1073/pnas.97.26.14200

Figure 4.

Figure 4

hTFIIIB50 is specifically required for RNA polymerase III transcription of genes with upstream promoter elements. (Upper) Transcription of the 7SK gene. (Lower) Transcription of the human tRNAMET gene. System A was comprised of 1.5 μl cEDF 1 M fraction, 40 ng rhTBP, and 24 ng rhTFIIIB150. System B consisted of 1.5 μl cEDF 1 M fraction and 5 μl B-EDF 0.3 fraction (unpublished observations). The following fractions were added: lane 1, none; lanes 2 and 3, 5 and 10 μl cEDF 0.2, respectively; and lanes 4 and 5, 5 and 10 μl of flow-through (FT), respectively, of an anti-hTFIIIB50 antibody column onto which a cEDF 0.2 fraction had been loaded. Purification of the cEDF fractions and antibody depletion procedures are described in Materials and Methods.