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. 2000 Dec 12;97(26):14206–14211. doi: 10.1073/pnas.260503997

Figure 1.

Figure 1

Segregation of the RbcS2-A57V suppressor mutation in reciprocal crosses. DNA was purified from wild-type (lane 1) revertant R116–10C (rbcL-L290F/RbcS2-A57V) (lane 2), a tetrad of progeny from the cross R116–10C mt+ X wild-type mt− (lanes 3–6), and a tetrad of progeny from the cross wild-type mt+ X R116–10C mt− (lanes 7–10). By using a pair of oligonucleotides specific for the RbcS2 gene, an 818-bp sequence (bases 640-1457 relative to bases 1–1,267 of the coding region of RbcS2) was PCR amplified from each sample, digested with HaeIII, and separated on a 3.5% agarose gel. The RbcS2-A57V mutation eliminates a HaeIII site, increasing the size of a 189-bp fragment to 210 base pairs. Progeny in lanes 3 and 5 had temperature-conditional acetate-requiring phenotypes. Progeny in lanes 4, 6, and 7–10 had wild-type (photosynthesis-competent) phenotypes at 35°C.