Table 1.

Genetic analysis of photosynthesis-competent revertants of the temperature-conditional photosynthesis-deficient rbcL-L290F mutant

Cross	Tetrads (ac-pf)*			Map distance†
	PD	NPD	T
rbcL-L290F, RbcS2-N54S, +, mt+ × +, +, pf-2, mt−	10	13	15	20
(revertant R116-1B)   (wild type)
rbcL-L290F, RbcS2-A57V, +, mt+ × +, +, pf-2, mt−	6	7	8	19
(revertant R116-10C)   (wild type)

^*All progeny receive the temperature-conditional chloroplast rbcL-L290F mutation, but only two in a tetrad receive the nuclear RbcS2 suppressor mutation. In the absence of the suppressor, progeny lack photosynthesis at 35°C and require acetate for growth (ac phenotype). Segregation of the ac phenotype was scored relative to the centromere-linked paralyzed flagella marker (pf phenotype), which allows parental-ditype (PD), nonparental-ditype (NPD), and tetratype (T) tetrads to be defined. 

^† Distances (% recombination × 100) between the RbcS2 suppressors and their centromeres were calculated as 100 × 0.5T/(PD + NPD + T).