Table 3.
Kinetic properties of Rubisco purified from wild-type, mutant rbcL-L290F, revertants rbcL-L290F/RbcS2-N54S and rbcL-L290F/RbcS2-A57V, and transformants RbcS1-Δ/RbcS2-N54S and RbcS1-Δ/RbcS2-A57V
| Enzymes | Ω* (VcKo/VoKc) | Vc* μmol⋅h−1⋅mg−1 | Kc* μM CO2 | Ko* μM CO2 | Vc/Kc† | Ko/Ko† | Vc/Vo† |
|---|---|---|---|---|---|---|---|
| Wild type | 60 ± 1 | 138 ± 13 | 32 ± 3 | 544 ± 38 | 4.3 | 17 | 3.5 |
| L290F | 54 ± 1 | 47 ± 1 | 43 ± 2 | 763 ± 41 | 1.1 | 18 | 3.0 |
| L290F/N54S | 60 ± 3 | 87 ± 1 | 33 ± 4 | 715 ± 37 | 2.6 | 22 | 2.7 |
| L290F/A57V | 58 ± 1 | 92 ± 3 | 32 ± 1 | 674 ± 79 | 2.9 | 21 | 2.8 |
| Δ/N54S | 61 ± 1 | 99 ± 8 | 26 ± 2 | 485 ± 47 | 3.8 | 19 | 3.2 |
| Δ/A57V | 59 ± 1 | 84 ± 7 | 26 ± 3 | 489 ± 87 | 3.2 | 19 | 3.1 |
*
The values are the means ± SD (n − 1) of three separate enzyme preparations.
†
Calculated values.