Table 4.
Kinetic properties of Rubisco purified from wild-type and suppressor strains RbcS2-N54S and RbcS2-A57V
Enzymes | Ω (VcKo/VoKc) | Vc μmol⋅h−1⋅mg−1 | Kc μM CO2 | Ko μM O2 |
---|---|---|---|---|
Wild type | 61 ± 1 | 152 ± 8 | 34 ± 2 | 537 ± 44 |
N54S | 60 ± 1 | 149 ± 10 | 30 ± 2 | 521 ± 20 |
A57V | 62 ± 1 | 137 ± 21 | 29 ± 3 | 497 ± 51 |
The values are the means ± SD (n − 1) of three separate enzyme preparations.