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The pH dependence of the steady-state turnover number kcat (s−1) for the hydration of CO2 catalyzed by human CA XII determined by stopped-flow spectrophotometry at 25°C. Solutions contained 5 μM EDTA and 25 mM of one of the buffers listed in Materials and Methods. The solid line is a least-squares fit with a maximal value of kcat at (4.0 ± 1.7) × 105 s−1 and an apparent pKa of 8.0 ± 0.2.
