Skip to main content
. 2000 Dec 12;97(26):14218–14223. doi: 10.1073/pnas.250452997

Table 1.

Sequence and position of the primers used in PCR amplification, 5′ end mapping, GMSA, and footprinting

Name Sequence 5′ to 3′ Gene and position
N2 TCCAACCCTAACGATGCTG NAG1: +19 to +37
N6 CCAGATCTGCGTTGGAAAATATAGCTTG NAG1: Complementary to +7 to +24, with a 10-bp adapter
N7 TTAAACTCAGCAACATCCTCTGCGG NAG1: −497 to −473
N9 GTCGGATCCTACCAACACTATGC NAG1: −1,725 to −1,704
N10 GGTTGATGAGAAATTGGGGTATT NAG1: Complementary to −1 to −23
N11 GGGGATGACCCGGTTGGAAGG NAG1: Complementary to +120 to +102
N12 CCATGGCAATATATATATAGGC NAG1: Complementary to −200 to −179
N13 CCATGGCTGCCCACATCACTTGC NAG1: Complementary to −195 to −217
N16 CTCAAAAACGTGTTACATTTG NAG1: −139 to −119
N17 ATGTCATTTACTAGATTCAC DAC1: +1 to +20
N19 GCGGATGACAAATTCT DAC1: Complementary to +106 to +91
N30SspI CCCAGGTGCTAATATTTGC NAG1: −408 to −390

Bases different from the homologous sequence are in bold. Position +1 refers to translation start codon ATG.