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Secondary structure of wild-type E. coli tRNALys. D, dihydrouridine; mnm5s2U, 5-methylaminomethyl-2-thiouridine; t6A, N6-threonylcarbamoyladenosine; ψ, pseudouridine; m7G, 7-methylguanosine; X, putative 3-(3-amino-3-carboxypropyl) uridine modification. The undermodified variants used in this study contain U34 in place of mnm5s2U34 and A37 in place of t6A37.
