Figure 1.

Retrotranslocation and Degradation of Δgpαf-BOF

After pΔgpαf mRNA was translated in vitro in the presence of [³⁵S]Met, SRP, microsomes, and either ɛBOF-Lys-tRNA^Lys (lanes 1–9) or Lys-tRNA^Lys (lanes 10–12), the microsomes were pelleted and then added to solutions containing ATP and either reticulocyte lysate (cytosol, lanes 1–3), lysate lacking hemoglobin (cytosol-Hb, lanes 4–6 and 10–12), or no lysate (no cytosol, lanes 7–9). The average percentages of Δgpαf-BOF radioactivity (A) and BOF emission (B) remaining at 15 and 30 min (0 min = 100%) for each sample are shown. (n = 7; ±3%–7% for ³⁵S, ±2%–6% for BOF.) ^∗, Δgpαf-BOF; ^∗∗, pΔgpαf-BOF.