Figure 4.

Suppression of ABCC5 splice variants by RNA interference increases full length ABCC5 mRNA expression. ARPE-19 cells were transfected with siRNA.1 targeting ABCC5_SV1, ABCC5_SV2 and ABCC5_SV3 or siRNA.2 targeting ABCC5_SV1 and ABCC5_SV2. ARPE-19 transfected with a nonsense siRNA was used as a negative control. Total RNA isolated 8 h, 24 h and 48 h after transfection was used for qRT-PCR analysis. The respective expression values are shown by specific symbols. The expression level of cells transfected with nonsense siRNA was set at 100%. Bar graphs represent standard deviations of reactions performed in triplicate.