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The three macromolecular assemblies used as test particles: (A) the thermosome (℘ ≈16 nm) with an 8-fold rotational symmetry, (B) GroEL (℘ ≈15 nm) with a 7-fold rotational symmetry, and (C) the 20S proteasome (℘ ≈12 nm) with a 7-fold rotational symmetry. The particles were filtered to 2-, 4-, and 8-nm resolution (from left to right).
