Table 1.

Sequences of primers and probes used in PCR.

Gene	Sequences 5’ to 3’	Tm °C (bp)	Amplicon size	Accession No.
Eukaryotic 18S rRNA	Supplied by Applied Biosystems (ABS)		190	X_03205
ß-actin	Forward: AGCCATGTACGTAGCCATCCA	62.72	80	NM_031144
	Reverse: TCTCCGGAGTCCATCACAATG	63.36
	Probe: TGTCCCTGTATGGCCTCTGGTGCTACCAC	72.34
SREBP1c	Forward: GGAGCCATGGATTGCACATT	63.15	191	L_16995
	Reverse: AGGAAGGCTTCCAGAGAGGA	60.47
	Probe: ACATGCTTCAGCTCATCAACAACCAA	69.28
ChREBP	Forward: AAAGGCCTCAAGTTGCTATG	59.33	166	AB_074517
	Reverse: AGACAACAGCCTCAGGTTTC	59.29
	Probe: TCAGTATGTGCAACGGAGGAAGAGCC	70.3
PPARα	Forward: TGGAGTCCACGCATGTGAAG	63.31	113	NM_013196
	Reverse: TGTTCCGGTTCTTTTTCTGAATCT	62.4
	Probe: CTTCTTTCGGCGAACTATTCGGCTAAAGC	71.16
PPARγ	Forward: CACAATGCCATCAGGTTTGG	62.29	71	NM_013124
	Reverse: GCTGGTTATCACTGGAGATC	62.24
	Probe: CACAGGCCGAGAAGGAGAAGCTGTTG	71.89
ACC-1	Forward: CCATTGGTATTGGGGCTTAC	59.16	110	J_03808
	Reverse: CCGACCAAGGACTTTGTTG	59.12
	Probe: TGGGACAAAGAACCATCCAGGTTGA	69.61
FAS	Forward: GCATCATTGGGCACTCCTT	61.03	81	X_13415
	Reverse: GCTGCAAGCACAGCCTCT	60.46
	Probe: AGGTTGCCTGTGGCTATGCAGATGG	71.08
L-PK	Forward: CGTTTGTGCCACACAGATGCT	60.7	79	NM_0.13631
	Reverse: CATTGGCCACATCGCTTGTCT	61.9
	Probe: AGCATGATCACTAAGGCTCGACCA	69.8
MCAD	Forward: TGTGCCTACTGCGTGACAGA	61.67	81	NM_016986
	Reverse: TTCATCACCCTTCTTCTCTGCTT	61.6
	Probe: AGGCTCGATGTGGCGGGCATTAA	71.43
IRS-2	Forward: TGAGACCAAGTGGCATCGTT	61.67	142	AF_087674
	Reverse: CTCTTGGGCTCAGTGGGTAGA	61.71
	Probe: CAGACCCTTCCCAGCATGCAAGAGTA	70.27