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. 2006 Aug 3;576(Pt 2):519–531. doi: 10.1113/jphysiol.2006.109561

Figure 4. Effect of transmural stimulation in LMMP preparations.

Figure 4

To confirm the myenteric plexus was intact in preparations devoid of CM (i.e. LMMP preparations), single-shot transmural stimuli were applied to all preparations once few or no stretch-activated junction potentials were observed. A, schematic of the preparation where recordings were made from LM and CM cells simultaneously at the oral or anal cut ends of the preparation. B, under maintained circumferential stretch, no junction potentials were recorded in LM or CM cells. C and D, despite the relative lack of electrical activity in both muscles, single-shot stimuli (40 V, 0.5 ms) evoked an EJP in both the LM and CM at the oral end, and an IJP in both the LM and CM at the anal end of the tissue; responses in both muscles occurred at the same time. E and F, following fixation of the LMMP preparations NADPH-diaphorase histochemistry also revealed that our dissection procedures had left the myenteric plexus intact.