Figure 5. Impact of inhibition of endothelial NO synthase or additional NO formation on shear stress-dependent superoxide anion formation.

Human endothelial cells were exposed to short-term (2 h) or long-term (24 h) laminar shear stress (ss, 30 dyne cm⁻²) without or with the NO synthase inhibitor l-NAME (500 μmol l⁻¹), or the NO donor DETA-NO (500 μmol l⁻¹). Superoxide dismutase- and catalase-inhibited superoxide anion (·O₂⁻) formation was determined by cytochrome c reduction assay. Values are given as means ±s.e.m. as a percentage of control; n ≥ 3 each; *P < 0.05 versus control.