Figure 6. Endothelial NO synthase inhibitor prevents down-regulation of specific NAD(P)H oxidase subunits by shear stress.

Human endothelial cells were exposed to long-term (24 h) laminar shear stress (ss, 30 dyne cm⁻²) without or with NO synthase inhibitor L-NAME (500 μmol l⁻¹). The mRNA expression of NAD(P)H oxidase subunits gp91^phox (A) and p47^phox (B) was measured by standard calibrated competitive reverse transcription-polymerase chain reaction and normalized to 18S rRNA RT-PCR. Values are given as means ±s.e.m. as a percentage of control; n ≥ 5 each; *P < 0.05 versus control.