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. 2006 Sep 28;577(Pt 3):997–1007. doi: 10.1113/jphysiol.2006.120006

Figure 3. Effect of acute exercise on PTP1B protein levels, activity and PTP1B association with IRβand IRS-1.

Figure 3

A, PTP1B protein level in DIO and DIO + EXE rats were compared with control group. B, PTP1B assay was performed as described in the Methods. C, tissue extracts were immunoprecipitated (IP) with anti-IRβ followed by immunoblotting (IB) with anti-PTP1B antibody or anti-IRβ antibody (upper and lower panels). D, insulin-stimulated IRβ phosphorylation (•) and the IRβ–PTP1B association (□) were determined using IP with anti-IRβ and IB with anti-PY antibody and IP with anti-IRβ followed by IB with anti-PTP1B antibody. E, IP with anti-IRS-1 followed by IB with anti-PTP1B antibody to evaluated the IRS-1–PTP1B association (upper panel). Muscle extracts were also IP with anti-IRS-1 and IB with anti-IRS-1 antibody (lower panels). The results of scanning densitometry were expressed as arbitrary units. Bars represent means ±s.e.m. of n = 6–8 rats. *P < 0.05, versus control and #P < 0.05, DIO + EXE versus DIO.