Figure 4. Effect of acute exercise on IRS-1 serine phosphorylation, JNK activity, IκBα degradation and IRS-1 and JNK protein levels in muscle of controls, DIO and DIO + EXE rats.

Tissue extracts were immunoblotted (IB) with anti-IRS-1307 phosphoserine antibody (A upper panel), anti IRS-1 antibody (A lower panel), anti-phospho JNK antibody (B upper panel), anti-JNK antibody (B lower panel), antiphospho-c-Jun antibody (C), anti-IκBα antibody (D) and anti-SOCS3 (E) in control, DIO and DIO + EXE rats. The results of scanning densitometry were expressed as arbitrary units. Bars represent means ±s.e.m. of n = 6–8 rats. *P < 0.05, versus control and #P < 0.05, DIO + EXE versus DIO.