Skip to main content
. 2006 Oct 12;577(Pt 3):1021–1032. doi: 10.1113/jphysiol.2006.120972

Figure 1. AMPK heterotrimer composition and phosphorylation in human skeletal muscle.

Figure 1

Lysates were prepared from biopsies taken in the vastus lateralis muscle before and after 20 min of exercise at 80%V˙O2,peak (n = 11). From 400 μg of lysate AMPK α1 (A), α2 (B), phospho α-Thr-172 (C) or γ3 (D) was immunoprecipitated (ip). The figure shows representative blots of the IP, post-IP and pre-IP (lysate) in the rested and exercised state. One-eighth of the IP corresponding to 50 μg was loaded together with 20 μg of the post- and pre-IPs. The blotted membranes were analysed with anti-α1, -α2, -phospho α-Thr-172, -β2, -γ1 and -γ3 as indicated to the far right. The small arrow indicates IgG light and heavy chains on the blots.