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Lysates are from the vastus lateralis muscle before and after exercise for 30 s (n = 10), 120 s (n = 9) and 20 min (n = 11), respectively; 25 μg of lysate were run on SDS-PAGE and blotted for phosphorylation of Thr-172 α-AMPK. Results are means ±s.e.m., ***P < 0.001, difference between rest and exercise.
