Figure 5. AMPK phosphorylation in relation to AMPK γ3-associated activity.

Lysates are from the vastus lateralis muscle before and after exercise for 30 s (n = 10), 120 s (n = 9) and 20 min (n = 11), respectively. A, 25 μg of lysate were run on SDS-PAGE and blotted for Thr-172 phosphorylation of α-AMPK. These signal intensities were multiplied with the amount of Thr-172 phosphorylated α-AMPK co-immunoprecipitated with γ3 to get the level of phosphorylation associated with the γ3 trimers. B, relationship between the γ3-associated level of Thr-172 α-AMPK phosphorylation and the AMPK activity of α2/β2/γ3. •= 30 s trial (n = 10); ▴= 120 s trial (n = 9) and ▪= 20 min trial (n = 11). A significant coefficient was found with linear regression analysis (r² = 0.84, P < 0.001). Results are means ±s.e.m., ***P < 0.001, difference between rest and exercise, †††P < 0.001, significantly different from the 20 min trial.