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. 2006 Oct 12;577(Pt 3):1021–1032. doi: 10.1113/jphysiol.2006.120972

Figure 6. ACCβ phosphorylation in relation to AMPK γ3-associated activity.

Figure 6

Lysates are from the vastus lateralis muscle before and after exercise for 30 s (n = 10), 120 s (n = 9) and 20 min (n = 11), respectively. A, 25 μg of lysate were run on SDS-PAGE and blotted for phospho Ser-221 ACCβ. B, relationship between the level of ACCβ phosphorylation and the AMPK activity of α2/β2/γ3. •= 30 s (n = 10), ▴= 120 s (n = 9) and ▪= 20 min (n = 11). A significant coefficient was found with linear regression analysis (r2 = 0.65, P < 0.001). Results are means ±s.e.m., ***P < 0.001, difference between rest and exercise, †P < 0.05 and †††P < 0.001, significantly different from the 20 min trial.