Cumulative food intake 1, 3, 5 hours after injection of saline (blue square), 1μmol/kg ghrelin (green square), 1μmol/kg obestatin (dark triangle) or 1μmol/kg obestatin + ghrelin (red triangle) : Panel A : in 24 h refeeding fasted mice (n= 20, 5 in each group) ; Panel B : in ad libitum fed mice (n= 20, 5 in each group)
a: ghrelin vs saline, P<0.01 ; b : ghrelin vs obestatin, P<0.01 ; c : ghrelin vs obestatin + ghrelin, P<0.05 ; d : ghrelin vs obestatin, P<0.05
Panel C : Cumulative food intake 18 h post injection in 24 h fasted (left) and ad libitum fed (right) mice receiving saline (blue), 1μmol/kg ghrelin (green), 1μmol/kg obestatin (dark) or 1μmol/kg obestatin + ghrelin (red)
a : ghrelin vs saline, P<0.05 ; b : ghrelin vs obestatin, P=0.06