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SFM images of the assembled chromatin [at 50% relative humidity in tapping mode on a Nanoscope III (Digital Instruments)] after 1-min incubation. (A) Short DNA fragment, obtained by shearing λ-phage, in extract at 1:100 dilution. (B) T4 DNA in extract at 1:25 dilution (Inset: magnified view). A drop was diluted in a large amount of buffer, immediately deposited on mica, incubated 20 s to initiate adsorption of complexes, and spread with argon to favor extended conformations.
