Table 4.
Rates of acid gain following ion substitutions or additions of the inhibitors EIPA or DIDS. Comparisons between paired or grouped cover slips
Rate of gain of acid (μm s−1) | |||||
---|---|---|---|---|---|
1 | 2 | n | P | ||
Initial rates during 1st recording intervals in the buffer solution indicated | |||||
A | (1) NaCl−Hepes, (2) NaCl−HCO3 | 7.0 ± 0.7 | 8.8 ± 1.1 | 34 | 0.21 |
Comparison of 2nd recording intervals | |||||
B | Two successive periods of either; | 12.5 ± 2.7 | 15.3 ± 2.4 | 6 | 0.43 |
(1) NaCl−HCO3 | |||||
(2) NaCl−HCO3+ 250 μm DIDS | |||||
C | (1) NaCl−Hepes → NMDGCl−Hepes | 15 | 0.52 | ||
(2) NaCl−Hepes → NaCl−Hepes + 5 μm EIPA | 10.1 ± 1.0 | 11.0 ± 1.0 | 10 | unpaired | |
D | (1) NaCl−Hepes → NMDGCl−Hepes | 9.6 ± 1.1 | 33.6 ± 5.1 | 16 | < 0.001 |
(2) NaCl−HCO3→ NMDGCl−HCO3 | 18 | unpaired |